Journal: bioRxiv

Article Title: TCF1 lo CD8 T cells proliferate and persist autonomously in tumors

doi: 10.64898/2026.01.17.700120

Figure Lengend Snippet: A. Experimental scheme: naive TCR TAG (Thy1.1) were adoptively transferred into tamoxifen-treated AST;Cre-ER T2 (Thy1.2) mice. FTY720 treatment or vehicle was administered every other day beginning on day −1 for 6 days or day 28 for 3 weeks. EdU was administered for the final 3 days prior to harvest. TCR TAG were re-isolated on day 5 and day 49 from the livers for flow cytometric analysis. B. Representative histogram and summary plot of % CD3ε + cells in peripheral blood 24 hours after initiation of FTY720 (purple) or vehicle (black) treatment. ****, P <000.1 determined by unpaired, two-tailed Student’s t -test. C. TCR TAG cell numbers in the livers of vehicle- (open black bars) and FTY720 (purple bars)-treated mice. Each symbol represents an individual mouse, with ****, P <0.0001 determined by two-way ANOVA followed by post hoc Šídák’s multiple comparisons test. D. Representative histogram of EdU+ incorporation in TCR TAG T cells and summary plot of % EdU + from the livers of vehicle- and FTY720-treated AST;Cre-ER T2 mice. Each symbol represents an individual mouse, two-way ANOVA followed by post hoc Šídák’s multiple comparisons test. E. Left, representative dot plots of TCF1 and KI67 expression in EdU + TCR TAG from the livers of AST;CreER T2 mice at day 5 (top) and day 49 (bottom) following vehicle and FTY720 treatment. Right, summary plots of % TCF1 hi (top) and KI67 + (bottom) of EdU + TCR TAG . Each symbol represents an individual mouse, two-way ANOVA followed by post hoc Šídák’s multiple comparisons test. For B-E , n=4-5 mice per group and representative of two independent experiments.

Article Snippet: Fingolimod (FTY720) Hydrochloride (Selleckchem, Cat# S50002, CAS-No:162359-56-0) stock solution was prepared by dissolving FTY720 in dimethyl sulfoxide (DMSO; Sigma Aldrich, Cat# D8418, CAS-No: 67-68-5) to a concentration of 10mg/mL and stored at −80°C.

Techniques: Isolation, Two Tailed Test, Expressing

Journal: bioRxiv

Article Title: TCF1 lo CD8 T cells proliferate and persist autonomously in tumors

doi: 10.64898/2026.01.17.700120

Figure Lengend Snippet: A. Experimental scheme: B16-OVA were injected subcutaneously into B6 mice. FTY720 treatment was administered every other day beginning on day 13 for 8 days. EdU was administered for the final 3 days. Lymphocytes were harvested from the tumors of mice on day 21 for flow cytometric analysis. B. Representative histogram and summary plot of CD3ε + cells in peripheral blood 24 hours after initiation of FTY720 (purple) or vehicle (black) treatment. ****, P <000.1 determined by unpaired, two-tailed Student’s t -test. C. Representative dot plots showing gating of OVA tetramer + (OVA-TET + )/PD1 + endogenous CD8 T cells and summary plot of OVA-TET + PD1 + CD8 + T cell numbers recovered from vehicle- and FTY720-treated B16-OVA tumor-bearing mice. Each symbol represents an individual mouse, unpaired, two-tailed Student’s t -test. D. Representative dot plots of TCF1 expression and EdU incorporation in OVA-TET + PD1 + CD8 + T cells from the tumors of vehicle- and FTY720-B16-OVA tumor-bearing mice. E. Summary plots of the percentage of EdU + (left) and TCF1 hi (right) of OVA-TET + PD1 + CD8 + T cells. Each symbol represents an individual mouse, unpaired, two-tailed Student’s t -test. For B-E , n=5 mice per group and representative of two independent experiments.

Article Snippet: Fingolimod (FTY720) Hydrochloride (Selleckchem, Cat# S50002, CAS-No:162359-56-0) stock solution was prepared by dissolving FTY720 in dimethyl sulfoxide (DMSO; Sigma Aldrich, Cat# D8418, CAS-No: 67-68-5) to a concentration of 10mg/mL and stored at −80°C.

Techniques: Injection, Two Tailed Test, Expressing